Gadolinium chelates inhibit intracellular calcium signaling in rat trigeminal ganglion neurons similar to rat dorsal root ganglion neurons, but less effective

Objectives: Gadolinium (Gd3+) based MR contrast agents (GBMCA) are manufactured with a chelation process, where organic ligand molecules form stable complex around Gd3+. The aim of the present study was to investigate possible impact free gadolinium and GBMCA in ganglia (trigeminal dorsal root) neuron cell cultures on intracellular calcium (Ca2+) since these could dissociate lead release toxic Gd3+ ions metabolic conditions. Methods: Ganglia cells were studied for responses nonspecific depolarization by high KCl+ Fura 2 AM loaded neurons monitoring changes [Ca2+]i using microscopic digital image analysis system. molecular doses 0.1 mmol/kg prepared same pH temperature applied cells. Results: Application Gd3+, gadobenate dimeglumine, gadobutrol gadodiamide rat led significant decreases basal levels, while gadoterate meglumine administration did not have impact. As expected, more effective when compared tı GBMCA. While almost full gadodiamide, other GBMCAs had irreversible effects at different levels. neglectable GBMCAs, although it exhibited slightly gadodiamide-like effects. Conclusions: In conclusion, chelates released irreversibly reduced levels identical TG DRG